| Correct to Sunday, 7th March 2010. | Unclaimed goals: | -5 | 0 | 0 | -5 | |
| Pos | Player | Club | Lge | Cup | FAC | Total |
| 1 | Jemma Tewkesbury | Yeovil Town | 22 | 1 | 6 | 29 |
| 2 | Vicky Barter | Newquay AFC | 12 | 0 | 5 | 17 |
| 3 | Lindsey Roe | Yeovil Town | 14 | 1 | 1 | 16 |
| 4 | Chloe Ranger | Havant & Waterlooville | 13 | 0 | 3 | 16 |
| 5 | Charley Wilson | Havant & Waterlooville | 12 | 0 | 3 | 15 |
| 6 | Laura Bray | Winscombe & Forest GR | 14 | 0 | 0 | 14 |
| 7 | Laura Gough | Plymouth Argyle | 12 | 0 | 0 | 12 |
| 8 | Lola Garcia Sanchez | Cullompton Rangers | 12 | 0 | 0 | 12 |
| 9 | Anusia Rourke | Yeovil Town | 8 | 1 | 3 | 12 |
| 10 | Sarah Mansfield | Newquay AFC | 10 | 0 | 0 | 10 |
| 11 | Dawne Campbell | Chesham United | 9 | 0 | 0 | 9 |
| 12 | Laura Perrett | Plymouth Argyle | 9 | 0 | 0 | 9 |
| 13 | Kirsty Richardson | Forest Green Rovers | 7 | 0 | 1 | 8 |
| 14 | Ellis Hillman | Cullompton Rangers | 6 | 2 | 0 | 8 |
| 15 | Amy Wild | Yeovil Town | 7 | 0 | 0 | 7 |
| 16 | Josie Copp | Cullompton Rangers | 7 | 0 | 0 | 7 |
| 17 | Sam Stone | Havant & Waterlooville | 7 | 0 | 0 | 7 |
| 18 | Laura Morgan | Swindon Town | 6 | 0 | 1 | 7 |
| 19 | Jodie Prince | Havant & Waterlooville | 5 | 0 | 2 | 7 |
| 20 | Leeta Rutherford | Havant & Waterlooville | 6 | 0 | 0 | 6 |
| 21 | Kate Bowers | Chesham United | 5 | 1 | 0 | 6 |
| 22 | Kayleigh Nardiello | Yeovil Town | 4 | 1 | 1 | 6 |
| 23 | Chantelle Clark | Havant & Waterlooville | 5 | 0 | 0 | 5 |
| 24 | Dani Salt | Newquay AFC | 4 | 0 | 1 | 5 |
| 25 | Sian Williams | Chesham United | 3 | 1 | 1 | 5 |
| 26 | Anneka Nuttall | Chesham United | 4 | 0 | 0 | 4 |
| 27 | Charlotte King | Forest Green Rovers | 4 | 0 | 0 | 4 |
| 28 | Hannah Smye | Oxford City | 4 | 0 | 0 | 4 |
| 29 | Jess Longworth | Newquay AFC | 4 | 0 | 0 | 4 |
| 30 | Kate Cook | Forest Green Rovers | 4 | 0 | 0 | 4 |