| Correct to Sunday, 16th May 2010. | Unclaimed goals: | -32 | 0 | 0 | -32 | |
| Pos | Player | Club | Lge | Cup | FAC | Total |
| 1 | Jemma Tewkesbury | Yeovil Town | 39 | 1 | 6 | 46 |
| 2 | Laura Bray | Winscombe & Forest GR | 24 | 0 | 0 | 24 |
| 3 | Dawne Campbell | Chesham United | 20 | 0 | 0 | 20 |
| 4 | Lindsey Roe | Yeovil Town | 18 | 1 | 1 | 20 |
| 5 | Charley Wilson | Havant & Waterlooville | 17 | 0 | 3 | 20 |
| 6 | Vicky Barter | Newquay AFC | 12 | 0 | 5 | 17 |
| 7 | Chloe Ranger | Havant & Waterlooville | 13 | 0 | 3 | 16 |
| 8 | Laura Perrett | Plymouth Argyle | 15 | 0 | 0 | 15 |
| 9 | Kate Bowers | Chesham United | 14 | 1 | 0 | 15 |
| 10 | Lola Garcia Sanchez | Cullompton Rangers | 13 | 0 | 0 | 13 |
| 11 | Laura Gough | Plymouth Argyle | 12 | 0 | 0 | 12 |
| 12 | Sian Williams | Chesham United | 10 | 1 | 1 | 12 |
| 13 | Anusia Rourke | Yeovil Town | 8 | 1 | 3 | 12 |
| 14 | Steph Mobbs | Oxford City | 11 | 0 | 0 | 11 |
| 15 | Kirsty Richardson | Forest Green Rovers | 10 | 0 | 1 | 11 |
| 16 | Ellis Hillman | Cullompton Rangers | 9 | 2 | 0 | 11 |
| 17 | Charlotte King | Forest Green Rovers | 10 | 0 | 0 | 10 |
| 18 | Sarah Mansfield | Newquay AFC | 10 | 0 | 0 | 10 |
| 19 | Kayleigh Nardiello | Yeovil Town | 8 | 1 | 1 | 10 |
| 20 | Anneka Nuttall | Chesham United | 9 | 0 | 0 | 9 |
| 21 | Leeta Rutherford | Havant & Waterlooville | 9 | 0 | 0 | 9 |
| 22 | Tash Sandalls | Chesham United | 8 | 0 | 1 | 9 |
| 23 | Amy Wild | Yeovil Town | 7 | 0 | 0 | 7 |
| 24 | Josie Copp | Cullompton Rangers | 7 | 0 | 0 | 7 |
| 25 | Sam Stone | Havant & Waterlooville | 7 | 0 | 0 | 7 |
| 26 | Shantell Woodward | Chesham United | 7 | 0 | 0 | 7 |
| 27 | Laura Morgan | Swindon Town | 6 | 0 | 1 | 7 |
| 28 | Jodie Prince | Havant & Waterlooville | 5 | 0 | 2 | 7 |
| 29 | Chantelle Clark | Havant & Waterlooville | 5 | 0 | 0 | 5 |
| 30 | Hannah Smye | Oxford City | 5 | 0 | 0 | 5 |